Journal: eNeuro
Article Title: NeuroART: Real-Time Analysis and Targeting of Neuronal Population Activity during Calcium Imaging for Informed Closed-Loop Experiments
doi: 10.1523/ENEURO.0079-24.2024
Figure Lengend Snippet: Custom-built dual beam path microscope for imaging and holographic optogenetic stimulation. A fixed laser beam of 1,064 nm is used for photostimulation, and the imaging laser is tunable within the range, 680–1,300 nm. 2P images are captured and amplified using photomultiplier tubes in parallel to simultaneous wide-field imaging through Hamamatsu ImagEM X2 EMCCD camera. (L1, L2, L3, L4, lenses; PC, Pockels cell; HWP, half wave plate; SL, scan lens; TL, tube lens; PMTs, photomultiplier tubes; Obj, objective; SLM, spatial light modulator). A 25×/1.05 NA water immersion objective and Boulder Nonlinear Systems (1,536 × 1,536) SLM with voltage overdrive and thermoelectric heating are used in our photostimulation setup.
Article Snippet: Our imaging system is also equipped with simultaneous epifluorescence imaging with a Hamamatsu cooled ImagEM X2 EMCCD camera, using a 20×/1.05 NA air objective.
Techniques: Microscopy, Imaging, Amplification